Determination of Iron Content in Iron Deficiency Drugs by UV-Visible Spectrophotometer

Abstract

The objective of this work was to validate a simple, precise and accurate spectrophotometric method for the determination of iron in the iron deficiency drugs, namely are Feroglobin B12, Ferose-F and Ferose. The proposed method is based on the reaction of iron with ammonium thiocyanate after the wet digestion of the drugs under study with HNO₃ and H₂O₂.  Effects of pH, temperature, standing time and thiocyanate concentration on the determination of iron in drugs containing iron have been investigated. The λ_max was 430 nm and the molar absorptivity of 0.0399 L mol^-1 cm^-1. The linear regression was in the range 0.5 - 60 μg/mL for iron content in hemoglobin. The detection limit and the limit of quantification were found to be 0.040 and 0.122 µg mL^-1 for the iron respectively, and with a linear regression correlation coefficient of 0.998. Recovery measurements ranged from 99.63-100.20%. This method is simple and fast can be used for the determination of iron in the iron deficiency drugs in pharmaceutical laboratories.

DOI: http://dx.doi.org/10.17807/orbital.v9i3.953