Orbital - Vol. 7 No. 1 - January-March 2015
FULL PAPERS

Application of Surfactant Micellar Solutions as Extractants and Mobile Phases for TLC-Determination of Purine Bases and Doping Agents in Biological Liquids

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  • Daria Yedamenko,
  • Lidia Loginova
Daria Yedamenko
V. N. Karazin Kharkov national university, Kharkiv, Ukraine
Lidia Loginova
V. N. Karazin Kharkov National University, Kharkiv
Published April 2, 2015
Keywords
  • micellar TLC,
  • surfactant,
  • serum,
  • urine,
  • doping agents,
  • purine bases
    • ...More
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How to Cite
(1)
Yedamenko, D.; Loginova, L. Application of Surfactant Micellar Solutions As Extractants and Mobile Phases for TLC-Determination of Purine Bases and Doping Agents in Biological Liquids. Orbital: Electron. J. Chem. 2015, 7, 28-35.

Copyright (c) 2015 Orbital: The Electronic Journal of Chemistry

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Abstract

Separation of caffeine and its metabolites (theophylline and theobromine) and doping agents (spironolactone, propranolol, and ephedrine) and determination of caffeine in serum sample and propranolol and ephedrine in urine were studied on normal-phase thin layers (“Sorbfil-UV-254”). Aqueous organic solvents and aqueous micellar surfactant solutions were compared as the mobile phases for separation. The acceptable separation of purine bases and doping agents was achieved by micellar Thin Layer Chromatography and normal-phase Thin Layer Chromatography. Anionic surfactant solution with added 1-propanol was the best eluent as for caffeine, theophylline, and theobromine separation, as for doping agents. The best characteristics of caffeine extraction from serum, and propranolol and ephedrine from urine were achieved when micellar eluent based on non-ionic Tween-80 surfactant was used.

DOI: http://dx.doi.org/10.17807/orbital.v7i1.632

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