Orbital - Vol. 7 No. 1 - January-March 2015
FULL PAPERS

Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-glucan) and Mycelial Biomass by Botryosphaeria rhodina MAMB-05

Ellen Cristine Giese
1. State University of Londrina 2. Coordination for Metallurgical and Environmental Process, Center of Mineral Technology, CETEM
Andressa F. G. Sumiya
State University of Londrina
Dionísio Borsato
State University of Londrina
Robert F. H. Dekker
Biorefining and Biotechnology Consultancy
Aneli M. Barbosa
State University of Londrina
Published April 3, 2015
Keywords
  • exopolysaccharide,
  • mycelium biomass,
  • shake flask optimization,
  • response surface method
How to Cite
(1)
Giese, E. C.; Sumiya, A. F. G.; Borsato, D.; Dekker, R. F. H.; Barbosa, A. M. Evaluation of Fermentative Parameters for the Production of Botryosphaeran (a(1-->3;1-->6)-β-D-Glucan) and Mycelial Biomass by Botryosphaeria Rhodina MAMB-05. Orbital: Electron. J. Chem. 2015, 7, 36-43.

Abstract

Botryosphaeran is an exopolysaccharide (EPS) of the (1"3;1"6)-β-D-glucan type produced by the fungus Botryosphaeria rhodina MAMB-05. In attempts to enhance its extracellular production, the quantitative effects of different nitrogen sources, fermentation time, inoculum size, C/N ratio, and initial pH were evaluated on the yields of botryosphaeran. Ammonium nitrate was selected as the best nitrogen source in examining these effects using a 2³-factorial central-composite experimental design, and analysis by the response surface method. According to a factorial design, the most important variables influencing both botryosphaeran and mycelium biomass production was inoculum concentration and the time of growth. Under these conditions, optimum botryosphaeran production occurred at 88 h of growth, 0.88 g of mycelium L-1 of nutrient mediumat C/N ratio of 30, and resulted in a 5.2 g L-1 yield of EPS in the fermentation broth. For production of the mycelium biomass, the optimum condition occurred at the same time of growth and inoculum concentration; however the best C/N ratio was 75, and resulted in a yield of 33.8 g L-1 of biomass. The results also indicated that the maximal mycelial growth is not associated to the yields of botryosphaeran production by Botryosphaeria rhodina MAMB-05.

DOI: http://dx.doi.org/10.17807/orbital.v7i1.637