Perimidines Synthesis: a Simple and Effective Experiment of Organic Chemistry for Undergraduate Students

Abstract

Practical laboratory experiments are essential to connecting theory to practical application and providing important support for academic training; however, many curricula still lack effective practical activities. This work proposes a didactic laboratory experiment highlighting the organic synthesis of a heterocyclic perimidine, providing an integrative approach to concepts such as carbonyl group addition and substitution (imine formation), thin-layer chromatography (TLC), liquid-liquid extraction, recrystallization, and yield calculations. Furthermore, a comparison was made between water and glycerol as environmentally friendly (green) solvents. Characterization techniques such as melting point, spectroscopy NMR ¹H and ¹³C, vibrational spectroscopy (FTIR) and UV-Vis were also used for product identification and discussion. The experiment was designed for undergraduate students and offered an opportunity to explore a variety of concepts in organic chemistry while integrating sustainable practices with fundamental techniques, demonstrating its potential as an effective and engaging educational tool.